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Volume 40 Issue 6
December 2025
    Citation: Yu Zhang, Yueyue Duan, Xueliang Zhu, Lei Shi, Juan Zhang, Wenquan Mao, Luyao Li, Cong Yuan, Maowen Sun, Haixue Zheng, Qi Wang. An adenovirus-vectored recombinant vaccine confers passive immunity protection from swine acute diarrhea syndrome coronavirus challenge in neonatal mice .VIROLOGICA SINICA, 2025, 40(6) : 1037-1044.  http://dx.doi.org/10.1016/j.virs.2025.12.007
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    An adenovirus-vectored recombinant vaccine confers passive immunity protection from swine acute diarrhea syndrome coronavirus challenge in neonatal mice

    • a. State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;

    • b. Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610213, China;

    • c. Chengdu National Agricultural Science and Technology Center, Chengdu 610213, China

    • Corresponding author: Haixue Zheng, zhenghaixue@caas.cn
      Qi Wang, qiwang@caas.cn
    • Received Date: 22 August 2025
      Accepted Date: 05 December 2025
      Available online: 09 December 2025
    • Swine acute diarrhea syndrome coronavirus (SADS-CoV), an emerging bat-origin Alphacoronavirus with demonstrated zoonotic potential, poses a significant threat to swine health and has considerable economic implications. Currently, no licensed vaccines are available. We constructed a replication-deficient human adenovirus type 5 (Ad5) vectored vaccine candidate, rAd5-SADS-S, which expresses the SADS-CoV spike (S) glycoprotein. The rAd5-SADS-S vaccine elicited robust SADS-CoV-specific humoral immunity and potent cellular responses in both mice and pigs. Notably, rAd5-SADS-S conferred passive protection to neonatal mice against lethal SADS-CoV challenge. These findings establish a preclinical foundation for the development of SADS-CoV vaccines.
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      An adenovirus-vectored recombinant vaccine confers passive immunity protection from swine acute diarrhea syndrome coronavirus challenge in neonatal mice

        Corresponding author: Haixue Zheng, zhenghaixue@caas.cn
        Corresponding author: Qi Wang, qiwang@caas.cn
      • a. State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;
      • b. Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610213, China;
      • c. Chengdu National Agricultural Science and Technology Center, Chengdu 610213, China
      • Received Date: 22 August 2025
      • Accepted Date: 05 December 2025

      Abstract: Swine acute diarrhea syndrome coronavirus (SADS-CoV), an emerging bat-origin Alphacoronavirus with demonstrated zoonotic potential, poses a significant threat to swine health and has considerable economic implications. Currently, no licensed vaccines are available. We constructed a replication-deficient human adenovirus type 5 (Ad5) vectored vaccine candidate, rAd5-SADS-S, which expresses the SADS-CoV spike (S) glycoprotein. The rAd5-SADS-S vaccine elicited robust SADS-CoV-specific humoral immunity and potent cellular responses in both mice and pigs. Notably, rAd5-SADS-S conferred passive protection to neonatal mice against lethal SADS-CoV challenge. These findings establish a preclinical foundation for the development of SADS-CoV vaccines.

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