STUDY OF PROTEOLYTIC ENZYME ASSOCIATED WITH THE POLYHEDRAL PROTEIN OF Galleria mellonella NUCLEAR POLYHEDROSIS VIRUS
Abstract: Dissolve kinetics for polyhedral matrix protein of Galleria mellonella nuclear polyhedrosis virus was investigated with wide range of pH,diff- erent ionic strength and temperature.A proteolytic enzyme was confir- med with the viral polyhedron.Its optimum pH for dissolved polyhedron is 8.8. The enzymes were purified by precipitaton with(NH_4)_2SO_4 and chrom- atograph with Sephacryl in result a five fold increase of relative enzyme activity.It was lyopholyzed to be a white cotton-form.Three different active ...