THE AVAILABILITY OF MONOCLONAL ANTIBODY IN DETECTING HERPES SIMPLEX VIRUS ANTIGEN WITH ELISA
Abstract: A double sandwich ELISA has been developed to detect herpes simplex virus(HSV) antigen in 70 clinical specimens, A valuation on availability of Monoclonal Anti body (McAb) in rapidly detecting HSV antigen was set, The sensitivity of McAb ELISA was 20-30 TCID50/0.1ml. The concordance of detecting HSV between McAb ELISA and virus isolation in clinical specimens was 85%. The mixture of two McAbs was used, it might elevate the sensitivity of ELISA.