Citation: LI Fang-He, CHEN Hong-Yun, YU Zhi-Qun, LI Yu-Xue, HAO Lian-Jie. DETECTION OF IgM ANTI-HBc BY ENZYME LINKED IMMUNOSORBENT ASSAY WITH AVIDIN LABELLED PEROXIDASE AND BIOTIN LABELLED ANTI-HBc .VIROLOGICA SINICA, 1987, 2(3) : 28.

DETECTION OF IgM ANTI-HBc BY ENZYME LINKED IMMUNOSORBENT ASSAY WITH AVIDIN LABELLED PEROXIDASE AND BIOTIN LABELLED ANTI-HBc

  • Available online: 05 September 1987
  • A new immunoenzymatic assay was established with avidin labelled peroxidase(A-P) and biotin labelled anti-HBc(B-Anti-HBc)for deteeting IgM antibody to hepatitis B core antigen. A-P, B-Anti-HBc and antiserum to human μ chain for the assay were prepared by our laboratory, Aggregated normal rabbit IgG was added to the buffer solution employed as a dilution to overcome the influence of rheumatoid factor of the blood specimens The specificity of the method was proved by SPA absorption method and anti-HBc inhibit...

  • 加载中
  • 加载中

Article Metrics

Article views(3555) PDF downloads(812) Cited by()

Related
Proportional views

    DETECTION OF IgM ANTI-HBc BY ENZYME LINKED IMMUNOSORBENT ASSAY WITH AVIDIN LABELLED PEROXIDASE AND BIOTIN LABELLED ANTI-HBc

    • 1. Laboratory of Clinical Immunology
    • 2. Tongji Hospital

    Abstract: A new immunoenzymatic assay was established with avidin labelled peroxidase(A-P) and biotin labelled anti-HBc(B-Anti-HBc)for deteeting IgM antibody to hepatitis B core antigen. A-P, B-Anti-HBc and antiserum to human μ chain for the assay were prepared by our laboratory, Aggregated normal rabbit IgG was added to the buffer solution employed as a dilution to overcome the influence of rheumatoid factor of the blood specimens The specificity of the method was proved by SPA absorption method and anti-HBc inhibit...

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return