Citation: XIAO Cheng-Zu, ZHANG Ling-An, KONG Wei-Wei, WANG Hong-Xia. PILOT PRODUCTION OF HIGH-TITRE MOUSE INTERFERON WITH MICROCARRIER SUSPENSION CULTURE SYSTEM .VIROLOGICA SINICA, 1987, 2(4) : 43.

PILOT PRODUCTION OF HIGH-TITRE MOUSE INTERFERON WITH MICROCARRIER SUSPENSION CULTURE SYSTEM

  • Available online: 05 December 1987
  • With MC-1 type microcarrier at concentration of 5mg/ml, the mouse L929 cell line could attach and proliferate on them very well if the stirring speed was lower than 50r/m.When the cell density was inoculated about 30×10~4/ml,it could proliferate to 1×10~6/ml after 3 days generally. At this time,priming with 25 IU/ml mouse IFN about 12-24 h and then induced with NDV and superinduced with cycloheximide(20μg/ml) and actinomycin D(2μg/ml),the titer of IFN in the media would be 10~5IU/ml approximately, or 10~5IU...

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    PILOT PRODUCTION OF HIGH-TITRE MOUSE INTERFERON WITH MICROCARRIER SUSPENSION CULTURE SYSTEM

    • 1. Institute of Microbiogy and Epidemiology
    • 2. Academy of Military Medical Sciences

    Abstract: With MC-1 type microcarrier at concentration of 5mg/ml, the mouse L929 cell line could attach and proliferate on them very well if the stirring speed was lower than 50r/m.When the cell density was inoculated about 30×10~4/ml,it could proliferate to 1×10~6/ml after 3 days generally. At this time,priming with 25 IU/ml mouse IFN about 12-24 h and then induced with NDV and superinduced with cycloheximide(20μg/ml) and actinomycin D(2μg/ml),the titer of IFN in the media would be 10~5IU/ml approximately, or 10~5IU...

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