THE APPLICATION OF FLUORESCENT SUBSTRATE IN ENZYME LINKED IMMUNOSORBENT ASSAY
Abstract: The substrate, p-nitrophenolphosphate disodium (PNPP), of alkaline phosphatase has been commonly used in enzyme linked immunosorbent assay (ELISA).The fluorescent substrate, 4-methylumbelliferal phosphate, usedin the indirect ELISA procedure(FS-ELISA)was compared with the substrate PNPP used in same indirect ELISA procedure. The fluorescent reaction could be observed under the UV-light at 253.7nm emission wavelength when coating was done with 0.1ml suspension of cucumber mosaic virus at the minimum concentr...