Citation: YU Shan-Qian, ZHANG Ruo-Ping. THE APPLICATION OF FLUORESCENT SUBSTRATE IN ENZYME LINKED IMMUNOSORBENT ASSAY .VIROLOGICA SINICA, 1987, 2(4) : 69.

THE APPLICATION OF FLUORESCENT SUBSTRATE IN ENZYME LINKED IMMUNOSORBENT ASSAY

  • Available online: 05 December 1987
  • The substrate, p-nitrophenolphosphate disodium (PNPP), of alkaline phosphatase has been commonly used in enzyme linked immunosorbent assay (ELISA).The fluorescent substrate, 4-methylumbelliferal phosphate, usedin the indirect ELISA procedure(FS-ELISA)was compared with the substrate PNPP used in same indirect ELISA procedure. The fluorescent reaction could be observed under the UV-light at 253.7nm emission wavelength when coating was done with 0.1ml suspension of cucumber mosaic virus at the minimum concentr...

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    THE APPLICATION OF FLUORESCENT SUBSTRATE IN ENZYME LINKED IMMUNOSORBENT ASSAY

    • 1. Virology Laboratory

    Abstract: The substrate, p-nitrophenolphosphate disodium (PNPP), of alkaline phosphatase has been commonly used in enzyme linked immunosorbent assay (ELISA).The fluorescent substrate, 4-methylumbelliferal phosphate, usedin the indirect ELISA procedure(FS-ELISA)was compared with the substrate PNPP used in same indirect ELISA procedure. The fluorescent reaction could be observed under the UV-light at 253.7nm emission wavelength when coating was done with 0.1ml suspension of cucumber mosaic virus at the minimum concentr...

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