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Citation: GUO Pei-Xuan. Inhibition of Novobiocin, Coumermycin A, Oxolinic Acid, Nalidixic Acid, Ethidium Bromide and ATP Analogue on the Packaging of Φ29 DNA in Vitro [J].VIROLOGICA SINICA, 1988, 3(2) : 198.

Inhibition of Novobiocin, Coumermycin A, Oxolinic Acid, Nalidixic Acid, Ethidium Bromide and ATP Analogue on the Packaging of Φ29 DNA in Vitro

  • Novobiocin, Coumermycin A, Oxolinic acid, Nalidixic acid, ethidium bromide and ATP analogue γ-S-ATP inhibited the DNA packaging of bacteriophage Φ29 in vitro. Besides ATP analogue, the concentration of the chemicals for 50% inhibition were 5, 90, 167, 380 and 1.3μg per ml, respectively. The intermediates of DNA packaging accumulated when the ATP analogue was added to the defined in vitro DNA packaging system, indicating that ATP was required for the entire process of DNA translocation. This finding provides...

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    Inhibition of Novobiocin, Coumermycin A, Oxolinic Acid, Nalidixic Acid, Ethidium Bromide and ATP Analogue on the Packaging of Φ29 DNA in Vitro

    • 1. Division of Microbiology
    • 2. Department of Animal Husbandry and Veterinary Medicine
    • 3. South China Agricultural University

    Abstract: Novobiocin, Coumermycin A, Oxolinic acid, Nalidixic acid, ethidium bromide and ATP analogue γ-S-ATP inhibited the DNA packaging of bacteriophage Φ29 in vitro. Besides ATP analogue, the concentration of the chemicals for 50% inhibition were 5, 90, 167, 380 and 1.3μg per ml, respectively. The intermediates of DNA packaging accumulated when the ATP analogue was added to the defined in vitro DNA packaging system, indicating that ATP was required for the entire process of DNA translocation. This finding provides...

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