Citation: WEN Ping, FANG Yi-Lan. Application of Dot-blot DNA Hybridization in Detection and Typing of Herpes Simplex Viruses .VIROLOGICA SINICA, 1988, 3(3) : 278.

Application of Dot-blot DNA Hybridization in Detection and Typing of Herpes Simplex Viruses

  • Available online: 05 September 1988
  • HSV type-common(7.8Kb)and HSV-2type-specific(3.0kb)DNA fragments were selected out from cloned HSV-2 (333) DNA Bgl Ⅱ H_2 fragment, and labeled with ~(32)P as probes to detect and type 4 HSV standard strains and 24 cervical viral isolates by dot-blot hybrization. In the results, 2HSV-1 and 2 HSV-2 were unequivocally differentiated, and 21 out of 24 isolates were identified as HSV, in which 3 were typed as HSV-1 and 18 as HSV-2. These results were consistent with those of ELISA, morover, the identification ra...

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    Application of Dot-blot DNA Hybridization in Detection and Typing of Herpes Simplex Viruses

    • 1. Department of Microbiology
    • 2. Xian Medical Univcrsity

    Abstract: HSV type-common(7.8Kb)and HSV-2type-specific(3.0kb)DNA fragments were selected out from cloned HSV-2 (333) DNA Bgl Ⅱ H_2 fragment, and labeled with ~(32)P as probes to detect and type 4 HSV standard strains and 24 cervical viral isolates by dot-blot hybrization. In the results, 2HSV-1 and 2 HSV-2 were unequivocally differentiated, and 21 out of 24 isolates were identified as HSV, in which 3 were typed as HSV-1 and 18 as HSV-2. These results were consistent with those of ELISA, morover, the identification ra...

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