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Volume 03 Issue 3
June 1988
    Citation: ZHENG Ji-Shan, LIN Jian-Wen, LI Fa-Qing, ZHENG Qi-Ge, LE Mei-Zhao. Isolation of Hepatitis A Virus in Vitro Directly from Peripheral Blood Mononuclear Cell of Patients with Hepatitis A .VIROLOGICA SINICA, 1988, 3(3) : 285.
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    Isolation of Hepatitis A Virus in Vitro Directly from Peripheral Blood Mononuclear Cell of Patients with Hepatitis A

    • 1.

      Institute of Military Medicine

    • 2.

      Nanjing Command

    • 3.

      PLA

    • Available online: 05 September 1988
    • HAAg were detected from the samples of peripheral blood mononuelear leukocytesof patients with hepatitis A using IF technique. The positive samples weer frozen and thawed 3 times and inoculated into PLC/PRF/5 cell cultures. Two strains of HAV were isolated, The isolates proved to be HAV based on specific activity in serologic tests and morphology. The results of our studies suggested that HAV be present not only in the serum freely but also associated with leukocytes. It is a simple method to screen the sam...
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      Isolation of Hepatitis A Virus in Vitro Directly from Peripheral Blood Mononuclear Cell of Patients with Hepatitis A

      • 1. Institute of Military Medicine
      • 2. Nanjing Command
      • 3. PLA

      Abstract: HAAg were detected from the samples of peripheral blood mononuelear leukocytesof patients with hepatitis A using IF technique. The positive samples weer frozen and thawed 3 times and inoculated into PLC/PRF/5 cell cultures. Two strains of HAV were isolated, The isolates proved to be HAV based on specific activity in serologic tests and morphology. The results of our studies suggested that HAV be present not only in the serum freely but also associated with leukocytes. It is a simple method to screen the sam...

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