Citation: CHEN Yong-Xuan, XUE Bao-Di, LIU Feng-Quan. Studies on WMV-2 aud CMV Detection Using Protein A Sandwich ELISA Method .VIROLOGICA SINICA, 1988, 3(4) : 357.

Studies on WMV-2 aud CMV Detection Using Protein A Sandwich ELISA Method

  • Available online: 05 December 1988
  • A new form of indirect enzyme-linked immunosorbent assay (ELISA) has been used for detection of WMV-2 and CMV in watermelon plants. The method uses protein A in two applications to direct ELISA method. The first layer of protein A is a precoating layer for the coating antibody. The second layer of protein A is conjugated enzyme for detecting the second antibody layer. In practical application, Protein A sandwich ELISA (PAS-ELISA) was more sensitive and specific than the direct double antibody sandwich form ...

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    Studies on WMV-2 aud CMV Detection Using Protein A Sandwich ELISA Method

    • 1. Nanjing Agricultural University
    • 2. Dept. of Plant Protection

    Abstract: A new form of indirect enzyme-linked immunosorbent assay (ELISA) has been used for detection of WMV-2 and CMV in watermelon plants. The method uses protein A in two applications to direct ELISA method. The first layer of protein A is a precoating layer for the coating antibody. The second layer of protein A is conjugated enzyme for detecting the second antibody layer. In practical application, Protein A sandwich ELISA (PAS-ELISA) was more sensitive and specific than the direct double antibody sandwich form ...

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