Studies on WMV-2 aud CMV Detection Using Protein A Sandwich ELISA Method
Abstract: A new form of indirect enzyme-linked immunosorbent assay (ELISA) has been used for detection of WMV-2 and CMV in watermelon plants. The method uses protein A in two applications to direct ELISA method. The first layer of protein A is a precoating layer for the coating antibody. The second layer of protein A is conjugated enzyme for detecting the second antibody layer. In practical application, Protein A sandwich ELISA (PAS-ELISA) was more sensitive and specific than the direct double antibody sandwich form ...