Citation: TU Zheng-Jin, XU Shan-Qian, ZHANG Re-Ping, WANG Ming-Qi. Preparation and Immunological Properties of Monoclonal Antibodies to Ribgrass Mosaic Virus (RMVsh) .VIROLOGICA SINICA, 1988, 3(4) : 364.

Preparation and Immunological Properties of Monoclonal Antibodies to Ribgrass Mosaic Virus (RMVsh)

  • Available online: 05 December 1988
  • Using hybridoma techniques, seven hybridoma cell lines: 1H2, 7H1, 10H1, 11H2, 12H3, 17H6, 29H1, secreting monoclonal antibodies to Shanghai Isolate of Ribgrass Mosaic Virus(RMVsh)were prepared by fusion on myeloma cells SP2/O with spleen cells of BALB/c mouse immunized with RMVsh. The subclasses of these monoclonal antibodies were determinated by double antibody sandwich ELISA. 1H2, 7H1 and other monoclonal antibodies belong to IgG_3, IgG_(2b) and IgG_1 subclass, respectively. The ascitic fluids were obtain...

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    Preparation and Immunological Properties of Monoclonal Antibodies to Ribgrass Mosaic Virus (RMVsh)

    • 1. Department of Biology
    • 2. Fudan University

    Abstract: Using hybridoma techniques, seven hybridoma cell lines: 1H2, 7H1, 10H1, 11H2, 12H3, 17H6, 29H1, secreting monoclonal antibodies to Shanghai Isolate of Ribgrass Mosaic Virus(RMVsh)were prepared by fusion on myeloma cells SP2/O with spleen cells of BALB/c mouse immunized with RMVsh. The subclasses of these monoclonal antibodies were determinated by double antibody sandwich ELISA. 1H2, 7H1 and other monoclonal antibodies belong to IgG_3, IgG_(2b) and IgG_1 subclass, respectively. The ascitic fluids were obtain...

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