Citation: DONG Guan-Mu, LIU Wen-Xue, SHU Yong-Xin. Use of AntibOdy Sensitized Human O Red Blood Cells for Reverse Passive Hemagglutination Test(RPHA) and RPHA Inhibition Test(RPHI) for Detection of Antigen and Antibody .VIROLOGICA SINICA, 1989, 4(1) : 100.

Use of AntibOdy Sensitized Human O Red Blood Cells for Reverse Passive Hemagglutination Test(RPHA) and RPHA Inhibition Test(RPHI) for Detection of Antigen and Antibody

  • Available online: 05 March 1989
  • We have studied RPHA and RPHI test using anti-EHFV antibody sensitized human red blood cell for detecting EHF antigen and antibody. The antigen used in RPHI was successfully prepared with infected mouse brains extracted by pH 9.0 borate buffered saline(BBS), providing a method of simplicity for preparing antigen. This RPHA test can be used for detection on antigen content of EHF vaccine instead of ELISA, and for detection of EHF virus in tissue culture fluid instead of using IF technique for detecting EHF a...

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    Use of AntibOdy Sensitized Human O Red Blood Cells for Reverse Passive Hemagglutination Test(RPHA) and RPHA Inhibition Test(RPHI) for Detection of Antigen and Antibody

    • 1. National Institute for the Control of Pharmaceutical and Biological Products
    • 2. Beijing

    Abstract: We have studied RPHA and RPHI test using anti-EHFV antibody sensitized human red blood cell for detecting EHF antigen and antibody. The antigen used in RPHI was successfully prepared with infected mouse brains extracted by pH 9.0 borate buffered saline(BBS), providing a method of simplicity for preparing antigen. This RPHA test can be used for detection on antigen content of EHF vaccine instead of ELISA, and for detection of EHF virus in tissue culture fluid instead of using IF technique for detecting EHF a...

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