Citation: ZHOU Lv-Qian, XUE Cui-Feng, SUN Fu-Lin. Production and Elimination of Unspecific Bands in Agar-double Diffusion of Plant Viruses .VIROLOGICA SINICA, 1989, 4(1) : 109.

Production and Elimination of Unspecific Bands in Agar-double Diffusion of Plant Viruses

  • Available online: 05 March 1989
  • Phosphate is usually used in purifying plant viruses, but phosphate buffer can form unspecific bands with some hosts during purification by agar-double diffusion. Unspecific bands often interfere with the determination of specific bands in detection of the plant viruses. The absorbing method can be utilized to eliminate the interfering phenomenon.

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    Production and Elimination of Unspecific Bands in Agar-double Diffusion of Plant Viruses

    • 1. Wuhan Institute of Virology. Academia Sinica
    • 2. Wuhan

    Abstract: Phosphate is usually used in purifying plant viruses, but phosphate buffer can form unspecific bands with some hosts during purification by agar-double diffusion. Unspecific bands often interfere with the determination of specific bands in detection of the plant viruses. The absorbing method can be utilized to eliminate the interfering phenomenon.

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