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Volume 04 Issue 2
April 1989
    Citation: LIN Bi-Run, LI Ying-Jie, OU Yang-Ming-Hui, FAN Huai-Zhong. Preparation of Monoclonal Antibodies to Papaya Ringspot Virus and Application in Serotyping .VIROLOGICA SINICA, 1989, 4(2) : 172.
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    Preparation of Monoclonal Antibodies to Papaya Ringspot Virus and Application in Serotyping

    • 1.

      1.South China Agricultural University

    • 2.

      Guangzhou.

    • 3.

      The Frist Medicine University of PLA

    • Available online: 05 June 1989
    • Three stable hybridoma cell lines secreting monoclonal antibodies against papaya ringspot virus were produced by fusing spleen cells of mice immunized with papaya ringspot virus(PRV-1) to mouse myeloma cell lines Sp2/0-Ag14. of three hybridoma cell lines, the titer of 23H1 secreting monoclonal antibody was found to be the highest, when testing by ELISA method, the titer of 23H1 inascitie fluid was 1:76800. Three cell lines secreting monoclonal antibodies were specific for papaya ringspot virus, not reactive...
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      Preparation of Monoclonal Antibodies to Papaya Ringspot Virus and Application in Serotyping

      • 1. 1.South China Agricultural University
      • 2. Guangzhou.
      • 3. The Frist Medicine University of PLA

      Abstract: Three stable hybridoma cell lines secreting monoclonal antibodies against papaya ringspot virus were produced by fusing spleen cells of mice immunized with papaya ringspot virus(PRV-1) to mouse myeloma cell lines Sp2/0-Ag14. of three hybridoma cell lines, the titer of 23H1 secreting monoclonal antibody was found to be the highest, when testing by ELISA method, the titer of 23H1 inascitie fluid was 1:76800. Three cell lines secreting monoclonal antibodies were specific for papaya ringspot virus, not reactive...

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