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Volume 04 Issue 4
August 1989
    Citation: PENG Hai, SHEN Xue-Ren, WU Jian-Hua, GONG Zu-Xun. The cDNA of Assembly Origin Sequence in TMV Genome Inhibiting TMV Assembly in Vitro .VIROLOGICA SINICA, 1989, 4(4) : 427.
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    The cDNA of Assembly Origin Sequence in TMV Genome Inhibiting TMV Assembly in Vitro

    • 1.

      Shanghai Institute of Biochemistry

    • 2.

      Academia Sinica. Shanghai

    • Available online: 05 December 1989
    • A 20 nucleotides DNA fragment which is complementary with the assembly origin of TMV-RNA was synthesized. Instead of antisense RNA, the DNA fragment could hybridize with TMV-RNA in nitrocellulose dot hybridization and in mini-volume solution hybridization assays. Using RNA-cDNA complex and 20S aggregates of TMV coat protein, the TMV in vitro assembly reaction experiment, which was monitored by 310nm absorption spectrum and electron microscopy, showed that TMV in vitro assembly could not proceed in results o...
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      The cDNA of Assembly Origin Sequence in TMV Genome Inhibiting TMV Assembly in Vitro

      • 1. Shanghai Institute of Biochemistry
      • 2. Academia Sinica. Shanghai

      Abstract: A 20 nucleotides DNA fragment which is complementary with the assembly origin of TMV-RNA was synthesized. Instead of antisense RNA, the DNA fragment could hybridize with TMV-RNA in nitrocellulose dot hybridization and in mini-volume solution hybridization assays. Using RNA-cDNA complex and 20S aggregates of TMV coat protein, the TMV in vitro assembly reaction experiment, which was monitored by 310nm absorption spectrum and electron microscopy, showed that TMV in vitro assembly could not proceed in results o...

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