Citation: MA Ju-Fang, JIANG Hui-Hui, HU Shan-Lian, YAN Hui-Qin, LI Jie. Development of Method for the Detection of Enteroviruses from Clams .VIROLOGICA SINICA, 1990, 5(1) : 41.

Development of Method for the Detection of Enteroviruses from Clams

  • Available online: 05 March 1990
  • A procedure for concentrating and recovering enteroviruses from clams (Arca( Anadara)subcrenata Lischke)has been developed and evaluated. Viruses were efficiently adsorbed to homogenized clam tissue by adjusting the homogenate to pH4.5. After low-speed centrifugation, the viruses were eluted from the sediment with pH 9.5 0.05mol/L glycine-saline. Instead of using Cat-Floc in Johnson s method, AlCl_3 was used to remove cytotoxity from clam tissue homogenates. After low-speed centrifugation, viruses were conc...

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    Development of Method for the Detection of Enteroviruses from Clams

    • 1. School of Public Health
    • 2. Shanghai Medical University

    Abstract: A procedure for concentrating and recovering enteroviruses from clams (Arca( Anadara)subcrenata Lischke)has been developed and evaluated. Viruses were efficiently adsorbed to homogenized clam tissue by adjusting the homogenate to pH4.5. After low-speed centrifugation, the viruses were eluted from the sediment with pH 9.5 0.05mol/L glycine-saline. Instead of using Cat-Floc in Johnson s method, AlCl_3 was used to remove cytotoxity from clam tissue homogenates. After low-speed centrifugation, viruses were conc...

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