Characterization of Thymidine Kinase Activity Associated with the Replication of Trichoplusia ni Nuclear Polyhedrosis Virus
Abstract: Trichoplusia ni nuclear polyhedrosis virus(TnNPV) stimulated thymidine kinase(TK) activities in infected Spodoptera frugiperda cells. TK activities both in infected and uninfected cells are similar in their pH, Mg~(++) optima for assays and elution patterns on DEAE-cellulose and Cibacron-blue sepharose chromatography. The facts that TnNPV could replicate in TK~- S. frugiperda cells but no TK activity was detected in the infected cells implies that the virus-stimulated activity was not contributed by the vir...