Citation: ZHANG De-Li. Selection of Super Reproductive Parvovirus Strains for Vaccine Preparation by Alternate Cultivation in Cell Lines with Very Different Susceptibiilty .VIROLOGICA SINICA, 1990, 5(2) : 221.

Selection of Super Reproductive Parvovirus Strains for Vaccine Preparation by Alternate Cultivation in Cell Lines with Very Different Susceptibiilty

  • Available online: 05 June 1990
  • This study has succeeded in selecting two strains(S_(18), S_(36)) of MEV with reproductive titre up to HA8192~×-32768~× or TCID_(50) Log_(10)~.7-10.3) from 6 strains of MEV with the titre 2~×- 64~× by alternate cultivation of 22 passages in two feline kidney cell(FKC) lines of susceptibility F_(81) strain and insusceptibility C_(81) strain ever reported as yet. The systematic tests proved that SMEV_(18,36) strains selected at present by alternative cultivation are super reproductive with powerful and extens...

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    Selection of Super Reproductive Parvovirus Strains for Vaccine Preparation by Alternate Cultivation in Cell Lines with Very Different Susceptibiilty

    • 1. Economic Animal Virology and Immunology Laboratory

    Abstract: This study has succeeded in selecting two strains(S_(18), S_(36)) of MEV with reproductive titre up to HA8192~×-32768~× or TCID_(50) Log_(10)~.7-10.3) from 6 strains of MEV with the titre 2~×- 64~× by alternate cultivation of 22 passages in two feline kidney cell(FKC) lines of susceptibility F_(81) strain and insusceptibility C_(81) strain ever reported as yet. The systematic tests proved that SMEV_(18,36) strains selected at present by alternative cultivation are super reproductive with powerful and extens...

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