Citation: LI Kai-Ben, YANG Su, WU Ru-Jian, XU Jie, KE Chong. The Purification of Citrus Tristeza Virus .VIROLOGICA SINICA, 1990, 5(3) : 312.

The Purification of Citrus Tristeza Virus

  • Available online: 05 September 1990
  • This paper deals with the Purification method of citrus tristeza virus (CTV) from infected sweet orange(Citrus sinensis[L]. Osbeck). The frozen and infected tissues were homogenized with 0.1mol/L Tris-buffer pH8.4 which contains 0.15% TritonX-100. After several differential centrifugations including two times of precipitation with PEG (MW 6,000), the supernatant was centrifuged by incontinuous sucrose density gradient of 200—400mg/ml and 600 mg/ml at 30,000 r/m (Hitachi, RPT 40 rot) for 3 hours. Then the ba...

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    The Purification of Citrus Tristeza Virus

    • 1. Fujian Academy of Agricultural Sciences
    • 2. Fushou 350003

    Abstract: This paper deals with the Purification method of citrus tristeza virus (CTV) from infected sweet orange(Citrus sinensis[L]. Osbeck). The frozen and infected tissues were homogenized with 0.1mol/L Tris-buffer pH8.4 which contains 0.15% TritonX-100. After several differential centrifugations including two times of precipitation with PEG (MW 6,000), the supernatant was centrifuged by incontinuous sucrose density gradient of 200—400mg/ml and 600 mg/ml at 30,000 r/m (Hitachi, RPT 40 rot) for 3 hours. Then the ba...

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