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Volume 05 Issue 4
August 1990
    Citation: NIE Zi-Lin, YU Yong-Xin, YAO Zhi-Hui. Development of EHF Virus Semi-micro Methylcellulose Plaque Assay .VIROLOGICA SINICA, 1990, 5(4) : 357.
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    Development of EHF Virus Semi-micro Methylcellulose Plaque Assay

    • 1.

      National Institute for the Control of Pharmaceutical and Biological Products

    • 2.

      Beijing 100050

    • Available online: 05 December 1990
    • A new semi-micro plaque assay has been established for epidemic hemorrhagic fever (EHF)virus, using methvlcellulose as overlay tested on Vero-E_(?) and Vero cell cultures. All EHF virus strains, including six tested on Vero-E_(?) cells and three tested on Vero cells, could form clear plaques that could be neutralized by rabbit anti-EHF virus immune serum. The results also show that the sensitivity of this method is consistent with the method of semi-micro agarose Plaque assay previously reported. However, t...
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      Development of EHF Virus Semi-micro Methylcellulose Plaque Assay

      • 1. National Institute for the Control of Pharmaceutical and Biological Products
      • 2. Beijing 100050

      Abstract: A new semi-micro plaque assay has been established for epidemic hemorrhagic fever (EHF)virus, using methvlcellulose as overlay tested on Vero-E_(?) and Vero cell cultures. All EHF virus strains, including six tested on Vero-E_(?) cells and three tested on Vero cells, could form clear plaques that could be neutralized by rabbit anti-EHF virus immune serum. The results also show that the sensitivity of this method is consistent with the method of semi-micro agarose Plaque assay previously reported. However, t...

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