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Volume 05 Issue 4
August 1990
    Citation: HE Kong-Wang, LIN Ji-Huang, JIANG Jie-Yuan, Chen-Jiang-Ping, HUANG Bao-Shun, LI Bao-Tong, YANG Yu-Hua. Preperation and Application of Monoclonal Antibodies to Bovine Rotavirus .VIROLOGICA SINICA, 1990, 5(4) : 430.
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    Preperation and Application of Monoclonal Antibodies to Bovine Rotavirus

    • 1.

      Institute of Animal Husbandry and Veterinary Medicine

    • 2.

      Jiangsu Academy of Agricultural Sciences

    • 3.

      Nanjing 210014Department of Microbiology

    • Available online: 05 December 1990
    • Two monoclonal antibodies (McAb, 2C7 and 3C9) were obtained by fusion of SP 2/0 myeloma cells with spleen cells from Balb/c mice immunized with rotavirus (RV)of HN-7 strain isolated from diarrheal calf in Henan Province. All the two McAbs reacted with the common group antigen of A-group RV, because the titers of two ascite McAbs were up to 1: 10~5 or above by indirect ELISA with RV of Bovine HN-7, BRV007, BRV014 and NCDV, Porcine Na86, Simian SA-11 and Human Wa strain, but were≤ 1: 8 and≤1: 100 respectively...
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      Preperation and Application of Monoclonal Antibodies to Bovine Rotavirus

      • 1. Institute of Animal Husbandry and Veterinary Medicine
      • 2. Jiangsu Academy of Agricultural Sciences
      • 3. Nanjing 210014Department of Microbiology

      Abstract: Two monoclonal antibodies (McAb, 2C7 and 3C9) were obtained by fusion of SP 2/0 myeloma cells with spleen cells from Balb/c mice immunized with rotavirus (RV)of HN-7 strain isolated from diarrheal calf in Henan Province. All the two McAbs reacted with the common group antigen of A-group RV, because the titers of two ascite McAbs were up to 1: 10~5 or above by indirect ELISA with RV of Bovine HN-7, BRV007, BRV014 and NCDV, Porcine Na86, Simian SA-11 and Human Wa strain, but were≤ 1: 8 and≤1: 100 respectively...

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