Citation: ZHENG Ji-Shan, LI Fa-Qing, LIN Jian-Men, PAN Xiu-Zhen, TAN Wei-Guo. Experimental Studies on Serial Passage of HAV in Human Leukocytes and Propagation of HAV In Various Kinds of Leukocytes in Vitro .VIROLOGICA SINICA, 1991, 6(1) : 79.

Experimental Studies on Serial Passage of HAV in Human Leukocytes and Propagation of HAV In Various Kinds of Leukocytes in Vitro

  • Available online: 05 March 1991
  • This paper reports the experimental studies on propagation of HAV in the human mononuclear leukocytes in vitro. The mononuelear leukocytes were separated from the peripheral blood of health person and the umbilical blood of fetus using Ficoll-Hypaque technique. The NJ-3 strain of HAV was inoclated into the adherent cells in culture flask and the suspensions of mononuclear leukocytes. After incubation the cultures were examined by indirect IF technique. It was found that both the adherent and nonadherent mon...

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    Experimental Studies on Serial Passage of HAV in Human Leukocytes and Propagation of HAV In Various Kinds of Leukocytes in Vitro

    • 1. Institute of Military Medicine
    • 2. Nanking Command

    Abstract: This paper reports the experimental studies on propagation of HAV in the human mononuclear leukocytes in vitro. The mononuelear leukocytes were separated from the peripheral blood of health person and the umbilical blood of fetus using Ficoll-Hypaque technique. The NJ-3 strain of HAV was inoclated into the adherent cells in culture flask and the suspensions of mononuclear leukocytes. After incubation the cultures were examined by indirect IF technique. It was found that both the adherent and nonadherent mon...

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