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Volume 06 Issue 3
June 1991
    Citation: MI Ke-Yong, WANG Qiang, ZHANG Fang-Dong, XIANG Xiu-Cheng. cDNA Synthesis of BVDV RNA and the Research of Its Hybridization .VIROLOGICA SINICA, 1991, 6(3) : 242.
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    cDNA Synthesis of BVDV RNA and the Research of Its Hybridization

    • 1.

      Wuhan Institute of Virology

    • 2.

      Academia Sinica

    • Available online: 05 September 1991
    • The BVDV(Bovine viral diarrhea virus)RNA is ssRNA.After the virus was purified, the BVDV RNA was extracted hy phenol-chloroform-isoamyl aleohl. The DNA contamination in the extracted BVDV RNA was degraded by DNase l. BVDV RNA was further purified by oligo dTcellulose affinity chromotography and agarose gel eleetrophoresis The purified BVDV RNA was polyadenylated at the 3′end under the catalysis of poly A polymerase, eDNA was synthesized with reverse transcriptase using the poly A-tailed BVDV RNA as template...
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      cDNA Synthesis of BVDV RNA and the Research of Its Hybridization

      • 1. Wuhan Institute of Virology
      • 2. Academia Sinica

      Abstract: The BVDV(Bovine viral diarrhea virus)RNA is ssRNA.After the virus was purified, the BVDV RNA was extracted hy phenol-chloroform-isoamyl aleohl. The DNA contamination in the extracted BVDV RNA was degraded by DNase l. BVDV RNA was further purified by oligo dTcellulose affinity chromotography and agarose gel eleetrophoresis The purified BVDV RNA was polyadenylated at the 3′end under the catalysis of poly A polymerase, eDNA was synthesized with reverse transcriptase using the poly A-tailed BVDV RNA as template...

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