Detection of Barley Yellow Mosaic Virus Using F(ab )_2 -ELISA

Barley yellow mosaic virus(BaYMV) was routinely detected and diagnosed by the F(ab )_2 indirect enzyme-linked immunosorbent assaya F(ab )_2-ELISA in which (1) virus in ELISA buffer was trapped by F(ab )_2 fragments of speeifie IgG diluted in 1000 to 4000 with 0.05mol/L sodium carbonate coating buffer pH9.6 on microtiter plate, (2)trapped virus was detected by intact IgG diluted 1in 1000 with ELISA buffer, (3) positive reaction was identified using protein diluted A alkalinephosphatase diluted 1 in 1000 to 2...