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Volume 07 Issue 1
February 1992
    Citation: WANG You-Chun, LI He-Min, HU Zong-Han. Analysis of peptides of HBsAg and the Epitopes of "a" Determinant by Monoclonal Antihody .VIROLOGICA SINICA, 1992, 7(1) : 48.
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    Analysis of peptides of HBsAg and the Epitopes of "a" Determinant by Monoclonal Antihody

    • 1.

      National Institute for the Control of Pharmaceutical and Biological Products

    • 2.

      Beijing 100050

    • Available online: 05 March 1992
    • Five selected McAbs were tested for their binding to HBsAg polypeptidecomposition by using Western Blot. McAb B_6, B_9 and B_(12) combined polypeptideP24 of HBsAg, while McAb A_1 and B_(13) combined both polypeptide p24 andP27 of HBsAg. Using the five McAbs, the epitope density of "a" determinantof HBsAg derived from different sources was also studied with the second an-tibody method. The result showed that there was similar density of epitopesagainst five McAbs on plasma-derived HBsAg (PD HBsAg), CHO cell-...
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      Analysis of peptides of HBsAg and the Epitopes of "a" Determinant by Monoclonal Antihody

      • 1. National Institute for the Control of Pharmaceutical and Biological Products
      • 2. Beijing 100050

      Abstract: Five selected McAbs were tested for their binding to HBsAg polypeptidecomposition by using Western Blot. McAb B_6, B_9 and B_(12) combined polypeptideP24 of HBsAg, while McAb A_1 and B_(13) combined both polypeptide p24 andP27 of HBsAg. Using the five McAbs, the epitope density of "a" determinantof HBsAg derived from different sources was also studied with the second an-tibody method. The result showed that there was similar density of epitopesagainst five McAbs on plasma-derived HBsAg (PD HBsAg), CHO cell-...

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