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Volume 07 Issue 1
February 1992
    Citation: WU Yun-Tao, KE Li-Hua, CAI Yi-Quan. Identification of PINPV--DNA Recombinant Colonies with Sandwich Hybridization .VIROLOGICA SINICA, 1992, 7(1) : 63.
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    Identification of PINPV--DNA Recombinant Colonies with Sandwich Hybridization

    • 1.

      Wuhan Institute of Virology

    • Available online: 05 March 1992
    • The genome of the bacularvirus PINPV was digested completely into sevenfragment (A、B、C、D_1、D_2、E、F) with Hind Ⅲ. These fragments were ligated in vitro with vector pUC18 seperately and used to transform host strainE. coli TG_1. Recombinant colonies were identified with sandwich hybridization.Seven kinds of recombinant colonies containing the PINPV-DNA Hind Ⅲ fra-gments were obtained, but unfortunately the A fragment was less than theoriginal one.
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      Identification of PINPV--DNA Recombinant Colonies with Sandwich Hybridization

      • 1. Wuhan Institute of Virology

      Abstract: The genome of the bacularvirus PINPV was digested completely into sevenfragment (A、B、C、D_1、D_2、E、F) with Hind Ⅲ. These fragments were ligated in vitro with vector pUC18 seperately and used to transform host strainE. coli TG_1. Recombinant colonies were identified with sandwich hybridization.Seven kinds of recombinant colonies containing the PINPV-DNA Hind Ⅲ fra-gments were obtained, but unfortunately the A fragment was less than theoriginal one.

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