Cucumber Mosaic Virus Coat Protein Inside the Chloroplast of the Infected Tobacco

The intact chloroplasts were made through protoplasts. In the SDSPAGE-silver stained gel, one extra protein band, which had the same mobility and molecular weight as the cucumber mosaic virus coat protein (CMV-cp), was found in the diseased chloroplast comparative to the health chloroplast. In the immunoblot testing, the band was highly reactive to the antiserum against the free CMV-cp, while the healthy and diseased sap treated healthy chloroplasts had no reaction. The result provides an evidence for the C...