Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA
Abstract: The virulent and modified canine adenovirus type 1 (CAV-1) DNAs extracted from canine kidney cells (MDCK) 36 hrs postinfection were both analysed with restriction enzymes EcoR Ⅰ, BamH Ⅰ, Pst Ⅰ, Nsi Ⅰ, Sph Ⅰ and Hind Ⅲ. and no difference was found from the electrophoresis pattern between the two viruses. The modified CAV-1 DNA was digested completely with Pst Ⅰ and cloned into, plasmid pBluescript SK taking shotgun procedure. Through hybridization selection of photobiotin labeled CAV-1 DNA and Pst Ⅰ digestio...