The cDNA Synthesis, Molecular Cloning and Nucleotide Sequence of Potato Leafroll Virus Coat Protein Gene

A coat protein gene eDNA of a PLRV isolate infected commercial potato cultivar "Purple Flower White" was synthesized by reverse transcription followed by Polymerase Chain Reaction amplification with synthesized 20merand 30mer primers. The synthesized eDNA was cloned in plasmid pUC19 in JM103. The full length CP gene eDNA clones pLCP2 and pLCP4 were further identified by restriction mapping analysis and, the nucleotide sequence of cDNA cloned in pLCP2 was analyzed with ABI 370 DNA sequence Tests show that th...