Monoclonal Antibodies to Freesia Chlorotic Stripe Mosaic Virus and Their Characteristics of Binding to Antigenic Determinants on the Coat Protein

Using hybridoma techniques, six monoclonal antibodies (mAb), P3, 4A4 6B1, 7A6, 7D1 and 7D8 to Freesia chlorotic stripe mosaic virus ( FCSMV), were prepared. The titers of mAb were 1:10~2—1:10~6. MAb 7A6 and 7D1 were IgG1 subclass. 7D8 and 4A4 were IgG2a and lgG2b subclass, respectively, mAb P3 and 6B1 were of IgG3 subclass. The reactions of mAb to virus particles and denatural coat protein indicated that mAb P3, 4A4 and 6B1 were associated with different sequential epitopes of coat protein, and others were ...