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Volume 08 Issue 1
February 1993
    Citation: JIN Yu-Huai, JIN Shi-Xiang, WANG Yong-Xiang, WANG Yu-Kun, GU Bao-Liang. Studies on PCR for Safety-detection of Rabies Virus in Samples .VIROLOGICA SINICA, 1993, 8(1) : 114.
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    Studies on PCR for Safety-detection of Rabies Virus in Samples

    • 1.

      Department of Microbiology

    • 2.

      Hebei Medical College

    • 3.

      Shijiazhuang

    • Available online: 05 March 1993
    • Polymerase Chain Reaction (PCR) combined with Heat-inactivation is adopted to detect of rabies virus. Using a pair of primers specific for rabies virus, reverse transcription and PCR were performed. The amplified products were visualized after electrophoresis on 2% agarose gel, stained with ethidium bromide and exposed to ultroviolet light. It is shown that:(1) A DNA fragment corresponding in size to the distance between the two primers (329 bp) could be amplified from the nucleic acid extracted from the ra...
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      Studies on PCR for Safety-detection of Rabies Virus in Samples

      • 1. Department of Microbiology
      • 2. Hebei Medical College
      • 3. Shijiazhuang

      Abstract: Polymerase Chain Reaction (PCR) combined with Heat-inactivation is adopted to detect of rabies virus. Using a pair of primers specific for rabies virus, reverse transcription and PCR were performed. The amplified products were visualized after electrophoresis on 2% agarose gel, stained with ethidium bromide and exposed to ultroviolet light. It is shown that:(1) A DNA fragment corresponding in size to the distance between the two primers (329 bp) could be amplified from the nucleic acid extracted from the ra...

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