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Volume 08 Issue 2
April 1993
    Citation: WANG Fei, LI Gang, GUO Ri-Bo, KE Wei-Min, LUO Hui-Rong, LIAO Yu-Huang. Detection of Dengue Virus 2 Genome by Reverse Transcription—Polymerase Chain Reaction .VIROLOGICA SINICA, 1993, 8(2) : 138.
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    Detection of Dengue Virus 2 Genome by Reverse Transcription—Polymerase Chain Reaction

    • 1.

      Department of Infectious Diseases

    • 2.

      Sun Yat-sen University of Medical Sciences. Guangzhou 510630Guangzhon Health and Anti-epidemic station

    • 3.

      Guangzhou.

    • Available online: 05 June 1993
    • Reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of dengue virus 2 genome. Two primers specific for dengue virus 2 and conservative between geographical variants bracketed a 150 nucleotide base in the E gene, corresponding to bases 1437—1586 in the dengue 2 genomic RNA sequence. After digested with Hind Ⅲ, the products of the reaction was divided into two fragments, 111bp and 39bp, respectively. The amplified products and digested fragments were shown in the ethidium ...
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      Detection of Dengue Virus 2 Genome by Reverse Transcription—Polymerase Chain Reaction

      • 1. Department of Infectious Diseases
      • 2. Sun Yat-sen University of Medical Sciences. Guangzhou 510630Guangzhon Health and Anti-epidemic station
      • 3. Guangzhou.

      Abstract: Reverse transcription-polymerase chain reaction (RT-PCR) was developed for the detection of dengue virus 2 genome. Two primers specific for dengue virus 2 and conservative between geographical variants bracketed a 150 nucleotide base in the E gene, corresponding to bases 1437—1586 in the dengue 2 genomic RNA sequence. After digested with Hind Ⅲ, the products of the reaction was divided into two fragments, 111bp and 39bp, respectively. The amplified products and digested fragments were shown in the ethidium ...

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