Citation: CHEN Zhi-Nan, ZHENG Guan-Biao, LIN Kong-Xun, FAN Huai-Zhong, ZHANG Chun-Xia, TIAN Bo. Studies on Techniques of Detecting Papaya Ringspot Virus .VIROLOGICA SINICA, 1993, 8(2) : 164.

Studies on Techniques of Detecting Papaya Ringspot Virus

  • Available online: 05 June 1993
  • A sensitive indirect dot-ELISA was developed by using nitrocellulose membrance (NCM) instead of microplate, 3% casin as a blocking agent, horse-radish peroxidase conjugated protein A as an enzyme-labelled complex and a dilution by 100 folds of IgG from antiserum of rabbit to PRV-Ys strain as the working concentration. and was found to b workable for assessing an amount of PRV particles at ng level (ave.10ng/spot, at 37℃). A PRV-RNA cDNA molecular probe (the insert ca. 300bp) was successfully constructed wit...

  • 加载中
  • 加载中

Article Metrics

Article views(3582) PDF downloads(798) Cited by()

Related
Proportional views

    Studies on Techniques of Detecting Papaya Ringspot Virus

    • 1. Plant Protection Department
    • 2. South China Agriculture University Guangzhou
    • 3. 0642Institute of Microbiol.

    Abstract: A sensitive indirect dot-ELISA was developed by using nitrocellulose membrance (NCM) instead of microplate, 3% casin as a blocking agent, horse-radish peroxidase conjugated protein A as an enzyme-labelled complex and a dilution by 100 folds of IgG from antiserum of rabbit to PRV-Ys strain as the working concentration. and was found to b workable for assessing an amount of PRV particles at ng level (ave.10ng/spot, at 37℃). A PRV-RNA cDNA molecular probe (the insert ca. 300bp) was successfully constructed wit...

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return