Citation: ZHANG Dong-Hai. Preliminary Study on the Improved Antibody Capture ELISA for Detection of EHF Specific IgE,IgA and IgG Antibodies .VIROLOGICA SINICA, 1993, 8(3) : 207.

Preliminary Study on the Improved Antibody Capture ELISA for Detection of EHF Specific IgE,IgA and IgG Antibodies

  • Available online: 05 September 1993
  • In this study, an improved antibody capture ELISA method was established for the detection of EHF specific IgE, IgA and IgG antibodies. The anti-human IgE, IgA and IgG monoclonal antibodies were used as coated antibodies and Hantaan virus group specific McAb (A35, A25-1) were used as HRP conjugates. In order to simplify the procedure, shorter test time and promote the sensitivity, the EHF virus antigen and HRP-McAb were mixed up then added simultaneously. Thirty-nine EHF patient s sera were tested by this ...

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    Preliminary Study on the Improved Antibody Capture ELISA for Detection of EHF Specific IgE,IgA and IgG Antibodies

    • 1. Zibo Second Health School
    • 2. Zibo

    Abstract: In this study, an improved antibody capture ELISA method was established for the detection of EHF specific IgE, IgA and IgG antibodies. The anti-human IgE, IgA and IgG monoclonal antibodies were used as coated antibodies and Hantaan virus group specific McAb (A35, A25-1) were used as HRP conjugates. In order to simplify the procedure, shorter test time and promote the sensitivity, the EHF virus antigen and HRP-McAb were mixed up then added simultaneously. Thirty-nine EHF patient s sera were tested by this ...

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