Western Blot Analysis of Viral Proteins Of Adult Diarrhea Rotavirus
Abstract: Adult diarrhea rotavirus (ADRV) is known as only one of group B rotavirus that causes human diarrhea. The viral proteins were determined by SDS-PAGE of purified virions from infected feces as follows: VP1 (136K), VP2 (113K), VP3 (92K), VP4 (84K), VP5 (64K), VP6 (47K), VP7(41K). Western blot analysis was used to examine their immunological properties. The results showed that all structural polypeptides are antigenic, VP6 is most strongly one and contains a common group B anti- gen. The rtatio of the antibod...