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Volume 08 Issue 3
June 1993
    Citation: CHEN Qi-Nan, ZHENG Guan-Biao, LIN Kong-Xun, FAN Fu-Zhong. Preliminary Research on Replicaiton of Papaya Ringspot Virus(PRV)in Papaya Protoplasts .VIROLOGICA SINICA, 1993, 8(3) : 263-270.
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    Preliminary Research on Replicaiton of Papaya Ringspot Virus(PRV)in Papaya Protoplasts

    • 1.

      Plant Protection Department

    • 2.

      South-China Agricullure University

    • 3.

      Guangzhou

    • 4.

      0642

    • Available online: 05 September 1993
    • A concentration of 110~6 cells/ml of high viable protoplasts was obtained by using the following optimal concentrations for isolating papaya mesophyll cell protoplasts: 1.0% Macerozyme R-10, 1.5% cellulase Onozuka R-10 and 0.45 mol/L mannitol in the medium (pH5.5). The inoculation was successfully carried out by exposing 510~5 cells/ml of the protoplasts to the final mixture of 1 g/ml PRV-Ys (or Vb) strains and 1g/ml poly-L-orintine (MW200000). The preliminary results of the studies on behavior kinecti...
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      Preliminary Research on Replicaiton of Papaya Ringspot Virus(PRV)in Papaya Protoplasts

      • 1. Plant Protection Department
      • 2. South-China Agricullure University
      • 3. Guangzhou
      • 4. 0642

      Abstract: A concentration of 110~6 cells/ml of high viable protoplasts was obtained by using the following optimal concentrations for isolating papaya mesophyll cell protoplasts: 1.0% Macerozyme R-10, 1.5% cellulase Onozuka R-10 and 0.45 mol/L mannitol in the medium (pH5.5). The inoculation was successfully carried out by exposing 510~5 cells/ml of the protoplasts to the final mixture of 1 g/ml PRV-Ys (or Vb) strains and 1g/ml poly-L-orintine (MW200000). The preliminary results of the studies on behavior kinecti...

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