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Volume 08 Issue 3
June 1993
    Citation: DIAO Xiao-Li, TUN Jian-Hua, HUANG Chun-Nong, WENG Cheng-Hua, GONG Jie-Xun. Studies on the Purification and Biochemical Properties of BaYMV .VIROLOGICA SINICA, 1993, 8(3) : 296-300.
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    Studies on the Purification and Biochemical Properties of BaYMV

    • 1.

      Shanhai Institute of Biochemistry

    • 2.

      Academia Sinica

    • 3.

      Shanghai

    • 4.

      0031Departement of Biology

    • 5.

      Hangzhou University

    • Available online: 05 September 1993
    • The Barley Yellow Mosaic disease is an important crop disease spread in the suburbs of Shanghai and coastal arears in East China. A purification procedure of Shanghai isolate of BaYMV was developed using the combination method of organic solvent treatment,ultracentrifugation,sucrose and CsCl densi- ty gradient centrifugation. The isolate showed the existence of two peaks in particle-length distribution (300 nm and 600 nm). SDS-PAGE of purified virus preparation demonstrated one major band of 29kd. A minor b...
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      Studies on the Purification and Biochemical Properties of BaYMV

      • 1. Shanhai Institute of Biochemistry
      • 2. Academia Sinica
      • 3. Shanghai
      • 4. 0031Departement of Biology
      • 5. Hangzhou University

      Abstract: The Barley Yellow Mosaic disease is an important crop disease spread in the suburbs of Shanghai and coastal arears in East China. A purification procedure of Shanghai isolate of BaYMV was developed using the combination method of organic solvent treatment,ultracentrifugation,sucrose and CsCl densi- ty gradient centrifugation. The isolate showed the existence of two peaks in particle-length distribution (300 nm and 600 nm). SDS-PAGE of purified virus preparation demonstrated one major band of 29kd. A minor b...

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