Citation: LI Yue-Xi, TANG Jia-Qi, SHI Jiang, ZHAO Hua-Zhong, JIAO Ren-Liang. Expression of HCV Core Protein in E.c0fi and Its Application .VIROLOGICA SINICA, 1994, 9(1) : 18.

Expression of HCV Core Protein in E.c0fi and Its Application

  • Available online: 05 March 1994
  • The cloned HCV core protein gene was inserted into the plasmid vector pJLA502 at EcoRI site,and the recombinant plasmid was transformed into E,coli DH_(5a),After inducing 5hrs at 42℃,SDS-PAGE analysis showed that the expressed core protein is twenty percent of total protein of E.coliDH5a;the purified core protein by Sephadex G200 gel filtration and Sepharose-4B-IgG affinity chro-matography has excellent antigenicity and specificity,and is good for detecting anti-HCV.

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    Expression of HCV Core Protein in E.c0fi and Its Application

    • 1. Nanjing Military Medical Research Institute,Nanjing 210002

    Abstract: The cloned HCV core protein gene was inserted into the plasmid vector pJLA502 at EcoRI site,and the recombinant plasmid was transformed into E,coli DH_(5a),After inducing 5hrs at 42℃,SDS-PAGE analysis showed that the expressed core protein is twenty percent of total protein of E.coliDH5a;the purified core protein by Sephadex G200 gel filtration and Sepharose-4B-IgG affinity chro-matography has excellent antigenicity and specificity,and is good for detecting anti-HCV.

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