The Analysis on Esterascs from BsNPV-Infected Buzura suppreSSaria and Bs484 Ceils
Abstract: By the polyacrylamide gel electrophoresis(PAGE)and thin-layer scanning,the esterases of thefourth instar larvae of the Buzura suppressaria and Bs484 cells were investigated after infection with nuclear pelyhedrosis viruses,The amount of the zymograms for mid-gut esterases in diseased larvae wasfound to be less than that in the healthy ones during the course of the disease,The results showed thatthe changes in type and content of mid-gut esterases were investigated at 8 hours postinfection,and thecharlges became obvious as infective time went on,The changes in content of esterases for Bs484 cellswere determined at 3 hours postinfection with BsNPV,but the type of esterases had no changes.