The Inhibitory Effects of Quinolones on Hepatitis B Virus in Vitro

uinolones were studied for their ability to inhibit hepatitis B virus DNA replicatiun,HBsAg andHBeAg exporssion in a HBV-transfeeted eell line(2.2.15 cell).The survival cell rate was determinedby MTT assay.The result shows that ID50(the drug concentration that inhibits HBsAg or HBeAg secretion by 50%) were 110g/ml and 199g/ml with Pipemidic Acid,64g/ml and 111g/ml with Norfloxacin,93g/ml and 210g/ml with Ciprofloxacin,105g/ml and 217g/ml with Ofloxacin.CD_50(the drug concentration that reduces cell growth by 50%) were 219g/ml,90g/ml,181g/ml,160g/ml,reepectively.In the range of drug concentration selected,they partially or completelybleeked the production of HBV particles and its replicative interniediates,especially the seneration of suporcoiled viral DNA((sc DNA).