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Citation: MENG Qing, ZHANG He-Ling, SONG Ba-Fu, LONG Rui-Jie, GENG Ji-Yang, YANG Yong-Jia-. Preparation of High Titer Antiserum to Swee!Potato Feathery Mottle Virus(SPFMV) [J].VIROLOGICA SINICA, 1994, 9(2) : 151-156.

Preparation of High Titer Antiserum to Swee!Potato Feathery Mottle Virus(SPFMV)

  • weet potato feathery mottle virus(SPFMV)was inoculated to I.selosa to propagare by graftingand was purified wirh 0.2mol/L pH7.2 PBK.Further purification of SPFMV was conducted by sucrose cushion and sucrose density gradient centrifugation.Purified SPFMV had a OD260/280 ratio of1.25.Rilbbit was immuned with purifled viruses to prepare antiSerum.Antisera produced to purifiedvirus had a titer of 1:1096 in microprecipitin and ring precipitin tests.SPFMV in leaves of sweet potato and I.selosa were detected respectively by indirect enzyme-linked immunosorbent assay(ELISA)onnitreeellulose membranes.

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    Preparation of High Titer Antiserum to Swee!Potato Feathery Mottle Virus(SPFMV)

    • 1. Biology  

    Abstract: weet potato feathery mottle virus(SPFMV)was inoculated to I.selosa to propagare by graftingand was purified wirh 0.2mol/L pH7.2 PBK.Further purification of SPFMV was conducted by sucrose cushion and sucrose density gradient centrifugation.Purified SPFMV had a OD260/280 ratio of1.25.Rilbbit was immuned with purifled viruses to prepare antiSerum.Antisera produced to purifiedvirus had a titer of 1:1096 in microprecipitin and ring precipitin tests.SPFMV in leaves of sweet potato and I.selosa were detected respectively by indirect enzyme-linked immunosorbent assay(ELISA)onnitreeellulose membranes.

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