Researches on Two-step PCR Amplification and a Rapid Detection of I-IBV-DNA

specific,sensitive,fast and convenient HBV-DNA detection system has been developed by usin8 two-step PCR combining with a biotinylated oligonucleotide hybridization method.The specificityof the system was proved by hybridizing both ￣(32)P-labelled HBV-DNA and biotinylated oligonucleotideprobes with PCR preduets,We also explored the sensitivity of the system,which can detect as less asabout 1-2 HBV-DNA molecules.The convenience of the system was demonstrated by the simple twotemperature PCR stepe and fast preducts detection with biotinylated oliunucleotide probe,This systemcan be used for both labortltory and clinical detection of HBV-DNA.