Molecular Cloning and Nucleotide Sequence of the Coat Protein Gene from Garlic Mosaic Virus

They have isolated garlic mosaic virus(GarMV)from naturally infected garlic plants and synthesized various pattial cDNA fragments using the GarMV genomic RNA as template.The length of thecoat protein(CP)gene was estimated about 0.8￣0.9 kb based on the estimated molecular weight ofthe coat protein subunit by protein SDS-Polyacrylamide gel analysis,Several clones containing cDNAfragments larger than 2kb were searched for a 3'terminal cDNA fragment including the complete CPgene and noncoding region.One clone,pGM495 containing a 2.4kb fragment was identified to be related to G arMv genomic RNA by Northern blotting assay and to contain the 3'-terminal poly(A)tract by terminal sequencing analysis suggesting the presence of these regions.The cDNA fragment in pGM495 has been completely sequenced.It contains 2379bp and three u-nique restriction sites,EcoRI I Pst I and BamH I ,which are conistant with the primary reetriction en-donuclease mapping analysis.There are several in-frame stop cedons,the first stop codon TAA is