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Volume 10 Issue 2
April 1995
    Citation: LI Gang, YAO Ji-Lu, PENG Wen-Wei, LV Ling, WANG Bin. Molecular Cloning and Sequence of the HCV NS1 Genomic Region .VIROLOGICA SINICA, 1995, 10(2) : 120.
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    Molecular Cloning and Sequence of the HCV NS1 Genomic Region

    • 1.

      Department of Infectious Diseases

    • 2.

      Sun Yat-sen University of medical Sciences

    • Available online: 05 June 1995
    • The NS1 genomic region of the hepatitis C virus(HCV),derived from the serum of a patient withchronic NANB hepetitis in Guangdong province,was cloned and sequenced.HCV RNA extracted bythe powder-adsorption procedure was converted to cDNA by everse transcription with random primer.Polymerase chain reaction was performed with the primers which span the NS1 region,The amplifiedproducts were separated by electrophoresis on a low melting-point agarose gel and a portion of gel,cor-respondlng to the expected size,w...
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      Molecular Cloning and Sequence of the HCV NS1 Genomic Region

      • 1. Department of Infectious Diseases
      • 2. Sun Yat-sen University of medical Sciences

      Abstract: The NS1 genomic region of the hepatitis C virus(HCV),derived from the serum of a patient withchronic NANB hepetitis in Guangdong province,was cloned and sequenced.HCV RNA extracted bythe powder-adsorption procedure was converted to cDNA by everse transcription with random primer.Polymerase chain reaction was performed with the primers which span the NS1 region,The amplifiedproducts were separated by electrophoresis on a low melting-point agarose gel and a portion of gel,cor-respondlng to the expected size,w...

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