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Volume 10 Issue 2
April 1995
    Citation: XIANG Bing-Yi, YANG Yong-Feng, LIU Li-Bing, MA Wen-Yu, JIANG Chao-Zhun, SU Cheng-Zhi. Chemical Synthesis of an Oligonucleotide Probe and Its Detection of JEV .VIROLOGICA SINICA, 1995, 10(2) : 131.
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    Chemical Synthesis of an Oligonucleotide Probe and Its Detection of JEV

    • 1.

      Institute of Molecular Biology

    • 2.

      The Fourth Military Medical University

    • 3.

      Xi an 710032

    • Available online: 05 June 1995
    • probe(19 oligonucleotides)for JEV corresponding to the sequence from 11 to 29 nt of the 3 end of Japanese encephalitis virus genomic RNA was designed and syn thesized with ABI 381A DNAsynthesizer,The oligonucleotide probe was labeled with[α-(32)p]using the Klenow fragment.Thespecific radioactivity of the probe is 8.10-8.86x107cpm/μgDNA.Hybridization with this probeshowed that lpg of target DNA,or the viruses harvested from the C6/36 cell culture at 6-12 hoursafter infection with JEV,Could be detected.
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      Chemical Synthesis of an Oligonucleotide Probe and Its Detection of JEV

      • 1. Institute of Molecular Biology
      • 2. The Fourth Military Medical University
      • 3. Xi an 710032

      Abstract: probe(19 oligonucleotides)for JEV corresponding to the sequence from 11 to 29 nt of the 3 end of Japanese encephalitis virus genomic RNA was designed and syn thesized with ABI 381A DNAsynthesizer,The oligonucleotide probe was labeled with[α-(32)p]using the Klenow fragment.Thespecific radioactivity of the probe is 8.10-8.86x107cpm/μgDNA.Hybridization with this probeshowed that lpg of target DNA,or the viruses harvested from the C6/36 cell culture at 6-12 hoursafter infection with JEV,Could be detected.

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