Immunogenicity of Grass Carp Hemorrhage Virus Polypeptides
Abstract: The immunogenicity of the 11 polypeptides of GCHV873 were commnd by using neutralizationtest for the determination of the major protective antigen.The viral polypeptides labelled with FITCwere analyzed on a 10%polyacrylamide gel.The individual polypeptides were retrieved from the gelby electric elution,and were used for immunizing rabbits to prepare antisera.Each anti-polypeptide antiserum was titrated in a neutralization test.The neutralization titres of the andsera indicated that theneutralizing activity o...