Amino Acid Analysis of the Major Structural Polypeptides of Rabbit Haemorrhagic Disease Virus

Rabbit haemorrhagic disease virus(RHDV)was purified from infected liver by freezing and thawing,chloroform extraction,PEG 6 000 precipitation and Sepharose-4B chromatography.Six polypeptides ,with the molecular weights of 64.5kD,61.4kD, 57.3kD,54.0kD.29.2kD and 28.1kD weredetermined by immuno blotting to be viral structural polypeptides.The major strucrural polypeptide(64.5kD),purified electrophoretically was analysed by hydrochloricacid lysis and fourteen kinds ofamino acids were found,of which,the base,ne...