Further characterization of viral inclusion body in the autopsy tissues of patients suf- fered from hemorrhagic fever with renal syndrome

Immunocytochemistry and in situ hybridization were performed on the autopsy tissues of 39 fetal cases of patients with hemorrhagic fever with renal syndrome to search for viral in-clusion body(IB)and study its antigenic and nucleic acid characters.The tissues with IB were further observed by electron and immunoelectron microscope.Immunohistochemically,IB,a coarse cytoplasmic granule. was positive for anti-nucleocapsid and hemogglutinin antibody,but negative for anti-evelope protein antibody.It was found in 20 of 39 cases including 10 cases died at hypotensive phase,9 cases died at oliguric phase and 1 case died at diuretic phase of HFRS,and distributed in the renal distal convoluted and collecting tubular cells,hepatocytes mucosal and glandular epithelial cells of respiratory tract,alveolar sacs,gastroin-testinal tract,tonsil,salivary gland,pallcreas,prostate gland and pituitary gland,and seminif-erous epithelial cell of testis,etc.The similar granules with viral RNA positive were observed only in a small