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Citation: WANG Zhen-Dong, LIU Yu-Le, TUN Yuan-Hua, FANG De-Chun, TIAN Bo. Identification,eDNA cloning and sequencing of a virus causing tobacco veinal necrosis .VIROLOGICA SINICA, 1995, 10(3) : 248.

Identification,eDNA cloning and sequencing of a virus causing tobacco veinal necrosis

  • Available online: 05 September 1995
  • Identification , cDNA cloning and sequencing of a virus causing tobacco veinal necrosis were carried out during 1 990一1993. The obvious symptom of the infected tobacco was veinal necrosis. Aphid(Myzus persicae)was demonstrated as transmitting vector. The virus only in-fected 8 species of tobacco and Physalis floridana form solanaceae,out of 35 species from 13 families tested.It's thermal inactivation point was 50一55℃,dilution end point was 1 0(-3)-10(-4),and longevity in vitro was 48-72 hours. The virus particles were flexous rods with Sizeof 720×12nm. Scroll,pinwhell and boundle inclusion were found in cytoplasm of leaf tissues of infected tobacco. The virus could react with the antiserum of PVY ̄0 by immunoelec-trophoresis test.Purified virus had A_(280)/A_(260)ratio of 0. 82. Using a pair of primers specific to PVY ̄N,reverse transcription and PCR were performed.A specific fragment of 0. 80kb corre-sponding in size to the PVY ̄N coat protein gene was amplified. T he products of PCR were cloned in plasmid

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    Identification,eDNA cloning and sequencing of a virus causing tobacco veinal necrosis

    • 1. Department of Plant protection,Shengyang Agricultural University

    Abstract: Identification , cDNA cloning and sequencing of a virus causing tobacco veinal necrosis were carried out during 1 990一1993. The obvious symptom of the infected tobacco was veinal necrosis. Aphid(Myzus persicae)was demonstrated as transmitting vector. The virus only in-fected 8 species of tobacco and Physalis floridana form solanaceae,out of 35 species from 13 families tested.It's thermal inactivation point was 50一55℃,dilution end point was 1 0(-3)-10(-4),and longevity in vitro was 48-72 hours. The virus particles were flexous rods with Sizeof 720×12nm. Scroll,pinwhell and boundle inclusion were found in cytoplasm of leaf tissues of infected tobacco. The virus could react with the antiserum of PVY ̄0 by immunoelec-trophoresis test.Purified virus had A_(280)/A_(260)ratio of 0. 82. Using a pair of primers specific to PVY ̄N,reverse transcription and PCR were performed.A specific fragment of 0. 80kb corre-sponding in size to the PVY ̄N coat protein gene was amplified. T he products of PCR were cloned in plasmid

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