Citation: XIAO Huo-Gen, GAO Qiao-Wan, ZHANG Shu-Guang, WANG Zhen-Zhong. Studies on the Detection of Cueum ber M osaie Virus in M ieroprogation of Banana .VIROLOGICA SINICA, 1995, 10(3) : 254.

Studies on the Detection of Cueum ber M osaie Virus in M ieroprogation of Banana

  • Available online: 05 September 1995
  • The present study established the direct antigen coating form for indirect ELISA (DAC-FLISA)for the detection of cucumber mosaic virus(CMV)in banana tissue culture. With aproper phosphate buffer solution,i.e.0. 1mol/L phosphate buffer(pH7.2,including 1%sodium diethyldithiocarbaniate) for preparing the crude plant sap to be detected,it was possi-ble to increase the sensitivity of DAC一ELISA to a dilution of plant sap at a rate of 1/5120.Atotal of 1 287 samples of productive material for banana tissue culture were detected forCMV.2.8%of them were found to be infected with CMV.

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    Studies on the Detection of Cueum ber M osaie Virus in M ieroprogation of Banana

    • 1. Department of Plant Protection,South China Agricultural University, Guangzhou 510642

    Abstract: The present study established the direct antigen coating form for indirect ELISA (DAC-FLISA)for the detection of cucumber mosaic virus(CMV)in banana tissue culture. With aproper phosphate buffer solution,i.e.0. 1mol/L phosphate buffer(pH7.2,including 1%sodium diethyldithiocarbaniate) for preparing the crude plant sap to be detected,it was possi-ble to increase the sensitivity of DAC一ELISA to a dilution of plant sap at a rate of 1/5120.Atotal of 1 287 samples of productive material for banana tissue culture were detected forCMV.2.8%of them were found to be infected with CMV.

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